Abstract
Genetic tools enable a diverse array of experimental approaches to dissect the relationships between brain function and behavior. Many genetic tools have been developed for use in the fruit fly, Drosophila melanogaster, making it a powerful model system due to its rich behavioral repertoire on one hand and, on the other, the accessible size and scale of its highly stereotyped nervous system. The stereotypy of the fly brain, in particular, makes it possible to interrogate, in essence, the same cell types—genetically, morphologically, physiologically, molecularly, and behaviorally—across individual flies. In this chapter, we describe how genetic tools can be used to target specific cell types in the fly. We then illustrate how these tools can be used to measure or manipulate a cell type’s activity, thereby shedding light on that cell type’s function within an intact circuit. Finally, we describe how tools that directly manipulate genes in a cell-type-specific manner can be used to identify the molecular and cellular mechanisms underlying each cell’s function.
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Esch, J.J., Fisher, Y.E., Leong, J.C.S., Clandinin, T.R. (2015). Genetic Pathways to Circuit Understanding in Drosophila. In: Arenkiel, B. (eds) Neural Tracing Methods. Neuromethods, vol 92. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1963-5_12
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