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Development of a Bacterial Nanoparticle Vaccine

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Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1225))

Abstract

A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN®).

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References

  1. Cozzi R, Scarselli M, Ferlenghi I (2013) Structural vaccinology: a three-dimensional view for vaccine development. Curr Top Med Chem 13:2629–2637

    Article  PubMed  Google Scholar 

  2. Mashburn-Warren LM, Whiteley M (2006) Special delivery: vesicle trafficking in prokaryotes. Mol Microbiol 61:839–846

    Article  PubMed  CAS  Google Scholar 

  3. Hodges K, Hecht G (2012) Interspecies communication in the gut, from bacterial delivery to host-cell response. J Physiol 590:433–440

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  4. Fahie M, Romano FB, Chisholm C et al (2013) A non-classical assembly pathway of escherichia coli pore-forming toxin cytolysin A. J Biol Chem 288:31042–31051

    Article  PubMed  CAS  Google Scholar 

  5. Shen Y, Giardino Torchia ML, Lawson GW et al (2012) Outer membrane vesicles of a human commensal mediate immune regulation and disease protection. Cell Host Microbe 12:509–520

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  6. Ochoa-Reparaz J, Garcia B, Solano C et al (2005) Protective ability of subcellular extracts from Salmonella Enteritidis and from a rough isogenic mutant against salmonellosis in mice. Vaccine 23:1491–1501

    Article  PubMed  CAS  Google Scholar 

  7. Ochoa J, Irache JM, Tamayo I et al (2007) Protective immunity of biodegradable nanoparticle-based vaccine against an experimental challenge with Salmonella Enteritidis in mice. Vaccine 25:4410–4419

    Article  PubMed  CAS  Google Scholar 

  8. Reed SG (2013) Vaccine adjuvants. Expert Rev Vaccines 12:705–706

    Article  PubMed  CAS  Google Scholar 

  9. Irache JM, Esparza I, Gamazo C et al (2011) Nanomedicine: novel approaches in human and veterinary therapeutics. Vet Parasitol 180:47–71

    Article  PubMed  Google Scholar 

  10. Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  11. Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234:1971–1975

    PubMed  CAS  Google Scholar 

  12. Osborn MJ (1963) Studies on the gram-negative cell wall. I. Evidence for the role of 2-Keto- 3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proc Natl Acad Sci U S A 50:499–506

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  14. Fairbanks G, Steck TL, Wallach DF (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617

    Article  PubMed  CAS  Google Scholar 

  15. Merril CR, Switzer RC, Van Keuren ML (1979) Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. Proc Natl Acad Sci U S A 76:4335–4339

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Tsai CM, Frasch CE (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115–119

    Article  PubMed  CAS  Google Scholar 

  17. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350–4354

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  18. Arbos P, Wirth M, Arangoa MA et al (2002) Gantrez AN as a new polymer for the preparation of ligand-nanoparticle conjugates. J Control Release 83:321–330

    Article  PubMed  CAS  Google Scholar 

  19. Camacho AI, Souza-Reboucas J, Irache JM et al (2013) Towards a non-living vaccine against Shigella flexneri: from the inactivation procedure to protection studies. Methods 60:264–268

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

This work was supported by a FIS grant PI12/01358 Ministerio de Sanidad y Consumo/FIS from Spain.

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Authors and Affiliations

  1. Department of Microbiology, University of Navarra, C/Irunlarrea no 1, 31008, Pamplona, Spain

    Carlos Gamazo, Ibai Tamayo & Ana Camacho

  2. Center for Nanomedicine, Sanford-Burnham Medical Research Institute, University of California, Santa Barbara, Santa Barbara, CA, 93106, USA

    Javier Ochoa-Repáraz

  3. Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31008, Pamplona, Spain

    Juan M. Irache

Authors
  1. Carlos Gamazo
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  2. Javier Ochoa-Repáraz
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  3. Ibai Tamayo
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  4. Ana Camacho
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  5. Juan M. Irache
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Corresponding author

Correspondence to Carlos Gamazo .

Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA

    Heide Schatten

  2. Cancer Research Center, University of Missouri, Columbia, Missouri, USA

    Abraham Eisenstark

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Gamazo, C., Ochoa-Repáraz, J., Tamayo, I., Camacho, A., Irache, J.M. (2015). Development of a Bacterial Nanoparticle Vaccine. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 1225. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1625-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1625-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1624-5

  • Online ISBN: 978-1-4939-1625-2

  • eBook Packages: Springer Protocols

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