Abstract
In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.
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Acknowledgment
We thank Tanja Strini for excellent technical assistance. This work was supported by the Netherlands Organization for Scientific Research (NWO; NGI Horizon grant 93511016).
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van der Eerden, B.C. et al. (2015). Identification of microRNAs in Human Plasma. In: Westendorf, J., van Wijnen, A. (eds) Osteoporosis and Osteoarthritis. Methods in Molecular Biology, vol 1226. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1619-1_7
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DOI: https://doi.org/10.1007/978-1-4939-1619-1_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1619-1
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