Abstract
Protein blotting is often used for identification and characterization of proteins on a membrane to which proteins separated by gel electrophoresis are transferred. The transferring process is sometimes problematic, in particular, for mucins and proteoglycans. Here, we describe a novel membrane electrophoresis technique, termed supported molecular matrix electrophoresis (SMME), in which a porous polyvinylidene difluoride (PVDF) membrane filter is used as the separation support. Proteins separated by this method can be immunoblotted without any transferring procedures.
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Acknowledgements
This work was performed as a part of the R&D project of the Industrial Science and Technology Frontier Program supported by the New Energy and Industrial Technology Development Organization (NEDO). This work was also supported by a Grant-in-Aid for Scientific Research (KAKENHI 23310154) from the Japan Society for the Promotion of Science (JSPS).
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Matsuno, Yk., Kameyama, A. (2014). Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_28
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DOI: https://doi.org/10.1007/978-1-4939-1292-6_28
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1291-9
Online ISBN: 978-1-4939-1292-6
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