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Lectin-Based Glycomics: How and When Was the Technology Born?

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Lectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1200))

Abstract

Lectin-based glycomics is an emerging, comprehensive technology in the post-genome sciences. The technique utilizes a panel of lectins, which is a group of biomolecules capable of deciphering “glycocodes,” with a novel platform represented by a lectin microarray. The method enables multiple glycan–lectin interaction analyses to be made so that differential glycan profiling can be performed in a rapid and sensitive manner. This approach is in clear contrast to another advanced technology, mass spectrometry, which requires prior glycan liberation. Although the lectin microarray cannot provide definitive structures of carbohydrates and their attachment sites, it gives useful clues concerning the characteristic features of glycoconjugates. These include differences not only in terminal modifications (e.g., sialic acid (Sia) linkage, types of fucosylation) but also in higher ordered structures in terms of glycan density, depth, and direction composed for both N- and O-glycans. However, before this technique began to be implemented in earnest, many other low-throughput methods were utilized in the late twentieth century. In this chapter, the author describes how the current lectin microarray technique has developed based on his personal experience.

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Authors and Affiliations

  1. Research Center for Stem Cell Engineering, National Institute of Advance Industrial Science and Technology (AIST), Central-2, 1-1-1, Umezono, Tsukuba, Ibaraki, 305-8568, Japan

    Jun Hirabayashi

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  1. Jun Hirabayashi
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Correspondence to Jun Hirabayashi .

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  1. National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

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Hirabayashi, J. (2014). Lectin-Based Glycomics: How and When Was the Technology Born?. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_20

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  • DOI: https://doi.org/10.1007/978-1-4939-1292-6_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1291-9

  • Online ISBN: 978-1-4939-1292-6

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