Abstract
The zebrafish model organism is well suited to study the role of specific genes, such as hox genes, in embryogenesis and organ function. The ability to modulate the activity of hox genes in living zebrafish embryos represents a cornerstone of such functional analyses. In this chapter we outline the basic methodology for nucleic acid injections into 1–2-cell-stage zebrafish embryos. We also report variations in this method to allow injection of mRNA, DNA, and antisense oligonucleotides to either overexpress, knock down, or knock out specific genes in zebrafish embryos.
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Acknowledgements
This work was supported by NIH grants NS038183 and HD065081 to CGS.
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Ladam, F., Sagerström, C.G. (2014). Functional Analysis of Hox Genes in Zebrafish. In: Graba, Y., Rezsohazy, R. (eds) Hox Genes. Methods in Molecular Biology, vol 1196. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1242-1_9
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DOI: https://doi.org/10.1007/978-1-4939-1242-1_9
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