Abstract
Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate the traction forces generated by engineered three-dimensional (3D) epithelial tissues embedded within a surrounding extracellular matrix (ECM). This technique provides insight into the physical mechanisms that underlie tissue morphogenesis in 3D.
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Abbreviations
- 2D:
-
Two-dimensional
- 3D:
-
Three-dimensional
- BSA:
-
Bovine serum albumin
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- DVC:
-
Digital volume correlation
- ECM:
-
Extracellular matrix
- EMT:
-
Epithelial-mesenchymal transition
- FBS:
-
Fetal bovine serum
- HBSS:
-
Hanks’ balanced salt solution
- PBS:
-
Phosphate-buffered saline
- PDMS:
-
Polydimethylsiloxane
- TFM:
-
Traction force microscopy
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Acknowledgements
We thank Lynn Loo for cleanroom access. This work was supported in part by grants from the NIH (GM083997 and HL110335), the David and Lucile Packard Foundation, the Alfred P. Sloan Foundation, and the Camille and Henry Dreyfus Foundation. C.M.N. holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. N.G. was supported in part by a Wallace Memorial Honorific Fellowship.
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Piotrowski, A.S., Varner, V.D., Gjorevski, N., Nelson, C.M. (2015). Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues. In: Nelson, C. (eds) Tissue Morphogenesis. Methods in Molecular Biology, vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_13
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DOI: https://doi.org/10.1007/978-1-4939-1164-6_13
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Online ISBN: 978-1-4939-1164-6
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