Abstract
Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes. Drawbacks to this technique mostly relate to proper handling of the specimen and the fluorophore-labeled antibodies. Therefore, having a basic understanding of fluorophores and antibodies is important for processing a specimen that yields a high signal-to-background ratio as well as troubleshooting problems, should they arise.
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Odell, I.D., Cook, D. (2014). Optimizing Direct Immunofluorescence. In: Day, C. (eds) Histopathology. Methods in Molecular Biology, vol 1180. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1050-2_6
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DOI: https://doi.org/10.1007/978-1-4939-1050-2_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1049-6
Online ISBN: 978-1-4939-1050-2
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