Abstract
Nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (SNPs) of Listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available. MLST has advantages over other methods as it can reconstruct ancestral and evolutionary linkage between L. monocytogenes isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.
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Acknowledgements
The authors wish to acknowledge the Institute Pasteur for providing the MLST database of L. monocytogenes in the Genotyping of Pathogens and Public Health Platform.
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Stessl, B., Rückerl, I., Wagner, M. (2014). Multilocus Sequence Typing (MLST) of Listeria monocytogenes . In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_6
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DOI: https://doi.org/10.1007/978-1-4939-0703-8_6
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