Abstract
The rapid emergence of multidrug-resistant pathogens has invoked concerns of our current limitations in controlling the spread of infectious disease. To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of P. aeruginosa. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microbe in multicellular systems.
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References
Chang W, Small DA, Toghrol F, Bentley WE (2005) Microarray analysis of Pseudomonas aeruginosa reveals induction of pyocin genes in response to hydrogen peroxide. BMC Genomics 6(1):115
Small DA, Chang W, Toghrol F, Bentley WE (2007) Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa. Appl Microbiol Biotechnol 76(5):1093–1105
Okuda J, Hayashi N, Okamoto M, Sawada S, Minagawa S, Yano Y, Gotoh N (2010) Translocation of Pseudomonas aeruginosa from the intestinal tract is mediated by the binding of ExoS to an Na, K-ATPase regulator, FXYD3. Infect Immun 78(11):4511–4522
Saeidi N, Wong CK, Lo T-M, Nguyen HX, Ling H, Leong SSJ, Poh CL, Chang MW (2011) Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol 7:521
Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343–345
Ling H, Saeidi N, Rasouliha BH, Chang MW (2010) A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Lett 584(15):3354–3358
Kang A, Chang MW (2012) Identification and reconstitution of genetic regulatory networks for improved microbial tolerance to isooctane. Mol Biosyst 8(4):1350–1358
Acknowledgements
This work was funded by the National Medical Research Council of Singapore (CBRG11nov109) and the National Research Foundation of Singapore (NRF-CRP5-2009-03). The first author wishes to dedicate this work to C.L.P., M.W.C., M.N., and S.T.
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Wong, C.K. et al. (2014). Therapeutic Microbes for Infectious Disease. In: Sun, L., Shou, W. (eds) Engineering and Analyzing Multicellular Systems. Methods in Molecular Biology, vol 1151. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0554-6_9
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DOI: https://doi.org/10.1007/978-1-4939-0554-6_9
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