Abstract
The acute murine lung infection model monitors Pseudomonas aeruginosa airway infections by multiple continuous and endpoint parameters. After intratracheal or intranasal infection it characterizes the course of infection via head-out spirometry, rectal temperature, weight loss, a body condition score based on nine physiological parameters, lung bacterial numbers, organ dissemination of bacteria, and a semiquantitative assessment of lung inflammation and further analysis. The generated data allows a robust classification of virulence of mutant or wild-type P. aeruginosa strains and/or of the susceptibility of wild-type or engineered mouse strains to infection. If standardized, the model is applicable to the preclinical assessment of antipseudomonal prevention and intervention strategies.
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References
Ramos J-L (ed) (2004) Pseudomonas. Kluwer Academic/Plenum, New York
Diamond SS, Cadwell GA (1979) Fatal bronchopneumonia and dermatitis caused by Pseudomonas aeruginosa in Atlantic Bottle-Nosed Dolphin. J Am Vet Med Assoc 175: 984–987
Wang SZ (1987) Diagnosis and control of a disease in mink caused by Pseudomonas aeruginosa. Chinese J Vet Med 13:16–17
JAX mice and services (2014) The Jackson Laboratory, Bar Harbor, Maine. http://jaxmice.jax.org/ Accessed 03 March 2014
Poch DS, Ost DE (2009) What are the important risk factors for healthcare-associated pneumonia? Semin Respir Crit Care Med 30:26–35
American Thoracic Society; Infectious Diseases Society of America (2005) Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia. Am J Respir Crit Care Med 171: 388–416
Klockgether J et al (2010) Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains. J Bacteriol 192:1113–21
Munder A et al (2011) Acute intratracheal Pseudomonas aeruginosa infection in cystic fibrosis mice is age-independent. Respir Res 12:148
Munder A et al (2002) Pulmonary microbial infection in mice: comparison of different application methods and correlation of bacterial numbers and histopathology. Exp Toxicol Pathol 54:127–33
Munder A et al (2005) Murine pulmonary infection with Listeria monocytogenes: differential susceptibility of BALB/c, C57BL/6 and DBA/2 mice. Microbes Infect 4: 600–611
Wölbeling F et al (2010) Head-out spirometry accurately monitors the course of Pseudomonas aeruginosa lung infection in mice. Respiration 80:340–346
Wölbeling F et al (2011) Lung function and inflammation during murine Pseudomonas aeruginosa airway infection. Immunobiology 216:901–908
Acknowledgement
This work was supported by a grant of the Deutsche Forschungsgemeinschaft to B. T. (SFB 587, project A9).
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Munder, A., Tümmler, B. (2014). Assessing Pseudomonas Virulence Using Mammalian Models: Acute Infection Model. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_59
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_59
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Publisher Name: Humana, New York, NY
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