Abstract
Uptake and excretion of nutrients is an integral part of a cell’s physiology. Using analytical chemistry techniques, metabolite uptake and excretion from the culture medium can be quantified. As cellular metabolism changes throughout growth, additional information is available if transient and growth phase-dependent changes are monitored. Here, we describe time-resolved metabolic footprinting (TReF), a technique which employs nuclear magnetic resonance spectroscopy and nonlinear curve fitting to understand and visualize metabolite utilization of P. aeruginosa.
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Acknowledgements
The authors thank Dr Olaf Beckonert and Dr Anthony Maher for technical assistance with NMR spectroscopy and Prof. Jeremy Nicholson and Prof. Elaine Holmes for support and access to facilities.
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Behrends, V., Williams, H.D., Bundy, J.G. (2014). Metabolic Footprinting: Extracellular Metabolomic Analysis. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_23
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_23
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