Abstract
Epigenetic regulation in the plant genome is associated with the determination of expression patterns of various genes. Methylation of DNA at cytosine residues is one of the mechanisms of epigenetic regulation and has been a subject of various studies. Various techniques have been developed to analyze DNA methylation, most of which involve isolation of chromatin from cells and further in vitro studies. Limited techniques are available for in situ study of DNA methylation in plants. Here, we present such an in situ method for DNA methylation analysis which has high sensitivity and good reproducibility.
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References
Vaillant I, Paszkowski J (2007) Role of histone and DNA methylation in gene regulation. Curr Opin Plant Biol 10(5):528–533
Cao X, Jacobsen SE (2002) Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes. Proc Natl Acad Sci U S A 99(Suppl 4):16491–16498
Mathieu O, Reinders J, Caikovski M, Smathajitt C, Paszkowski J (2007) Transgenerational stability of the Arabidopsis epigenome is coordinated by CG methylation. Cell 130(5):851–862
Widman N, Jacobsen SE, Pellegrini M (2009) Determining the conservation of DNA methylation in Arabidopsis. Epigenetics 4(2):119–124
Kathiria P, Sidler C, Golubov A, Kalischuk M, Kawchuk LM, Kovalchuk I (2010) Tobacco mosaic virus infection results in an increase in recombination frequency and resistance to viral, bacterial, and fungal pathogens in the progeny of infected tobacco plants. Plant Physiol 153(4):1859–1870
Luna E, Bruce TJ, Roberts MR, Flors V, Ton J (2012) Next-generation systemic acquired resistance. Plant Physiol 158(2):844–853
Slaughter A, Daniel X, Flors V, Luna E, Hohn B, Mauch-Mani B (2012) Descendants of primed Arabidopsis plants exhibit resistance to biotic stress. Plant Physiol 158(2):835–843
DeAngelis JT, Farrington WJ, Tollefsbol TO (2008) An overview of epigenetic assays. Mol Biotechnol 38(2):179–183
Mayer W, Niveleau A, Walter J, Fundele R, Haaf T (2000) Embryogenesis: demethylation of the zygotic paternal genome. Nature 403(6769):501–502
Naumann K, Fischer A, Hofmann I, Krauss V, Phalke S, Irmler K, Hause G, Aurich AC, Dorn R, Jenuwein T, Reuter G (2005) Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis. EMBO J 24(7):1418–1429
Oakeley EJ, Podesta A, Jost JP (1997) Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation. Proc Natl Acad Sci U S A 94(21):11721–11725
Manak JR, Wen H, Van T, Andrejka L, Lipsick JS (2007) Loss of Drosophila Myb interrupts the progression of chromosome condensation. Nat Cell Biol 9(5):581–587
Zink D, Fischer AH, Nickerson JA (2004) Nuclear structure in cancer cells. Nat Rev Cancer 4(9):677–687
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Kathiria, P., Kovalchuk, I. (2017). In Situ Analysis of DNA Methylation in Plants. In: Kovalchuk, I. (eds) Plant Epigenetics. Methods in Molecular Biology, vol 1456. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-4899-7708-3_7
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DOI: https://doi.org/10.1007/978-1-4899-7708-3_7
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Publisher Name: Humana Press, Boston, MA
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Online ISBN: 978-1-4899-7708-3
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