Abstract
Assessment of functional tumor-specific T-cell responses in preclinical tumor models represents an important tool for successful translation of new immunotherapies to clinics. Usually, it requires a known tumor antigen target. Here, we describe the method to detect tumor-specific T cell after immunotherapies without a known antigen. Splenocytes, lymph node immune cells, or PBMCs are isolated from treated mice and stimulated with relevant tumor cells ex vivo before immunospot analysis of Granzyme B and interferon γ-positive T cells. The method is especially valuable for monitoring tumor-specific T cells after vaccination with various whole tumor vaccines or after in situ vaccination and other antigen agnostic immunotherapies, where no specific antigens are used.
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Kamenšek, U., Remic, T. (2024). Immunospot Assessment of T-Cell Responses in Preclinical Tumor Models with Undefined Target Antigens. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_15
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DOI: https://doi.org/10.1007/978-1-0716-3714-2_15
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