Abstract
Studying salivary gland mucins is important for elucidating the pathogenesis of salivary gland diseases, including tumors and xerostomia, and developing diagnostic methods for them. Classic methods for isolating mucins from salivary glands require sacrificing several animals to obtain sufficient quantities of mucin and are time-consuming. Supported molecular matrix electrophoresis (SMME) was used to characterize mucins and their glycans. With this method, mucins can be analyzed within 2 days using less than 100 mg of tissue and without using expensive equipment, such as an ultracentrifuge. This chapter describes a method for preparing mucin solutions for SMME analysis of salivary gland mucins.
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References
Robinson L, van Heerden MB, Ker-Fox JG et al (2021) Expression of mucins in salivary gland mucoepidermoid carcinoma. Head Neck Pathol 15:491–502
Tettamanti G, Pigman W (1968) Purification and characterization of bovine and ovine submaxillary mucins. Arch Biochem Biophys 124:41–50
Oemrawsingh I, Roukema PA (1974) Isolation, purification and chemical characterization of mucins from human submandibular glands. Arch Oral Biol 19:615–626
Denny PA, Denny PC, Jenkins K (1980) Purification and biochemical characterization of a mouse submandibular sialomucin. Carbohydr Res 87:265–274
Matsuno Y-K, Saito T, Gotoh M et al (2009) Supported molecular matrix electrophoresis: a new tool for characterization of glycoproteins. Anal Chem 81:3816–3823
Kameyama A, Yamakoshi K, Watanabe A (2019) A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis. Biochim Biophys Acta Proteins Proteom 1867:76–81
Iida M, Matsuno YK, Watanabe A et al (2019) A sialo-oligosaccharide-rich mucin-like molecule specifically detected in the submandibular glands of aged mice. Arch Oral Biol 97:52–58
Kameyama A, Thet Tin WW, Nishijima R et al (2021) Alteration of mucins in the submandibular gland during aging in mice. Arch Oral Biol 121:104967
Kameyama A, Nishijima R, Yamakoshi K (2021) Bmi-1 regulates mucin levels and mucin O-glycosylation in the submandibular gland of mice. PLoS One 16:e0245607
Isaka E, Sugiura T, Hashimoto K et al (2021) Characterization of tumor-associated MUC1 and its glycans expressed in mucoepidermoid carcinoma. Oncol Lett 22:702
Sugiura T, Hashimoto K, Kikuta K et al (2023) Expression and localisation of MUC1 modified with sialylated core-2 O-glycans in mucoepidermoid carcinoma. Sci Rep 13:5752
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Sugiura, T., Kameyama, A. (2024). Preparation of Soluble Mucin Solutions from the Salivary Glands. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_3
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3669-5
Online ISBN: 978-1-0716-3670-1
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