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Creating a Reproducible Model of Spinal Cord Injury in Rats: A Contusion Approach

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Neuroprotection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2761))

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Abstract

Spinal cord injury (SCI) is a devastating clinical condition that affects millions of people worldwide. SCI primarily affects males in younger age groups. It is characterized by a complex of neurological dysfunctions that can lead to permanent disability. We describe an adapted technique for SCI, i.e., a contusion model of SCI, in this chapter. This model is widely used to study the pathology of SCI and test potential therapies. The experimental contusion is performed by using a compression device, which allows the creation of a reproducible injury animal model through the definition of specific injury parameters. A detailed methodology has been developed and described here that utilizes a stereotactic frame and impactor to produce reproducible injuries.

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References

  1. Alizadeh A, Dyck SM, Karimi-Abdolrezaee S (2019) Traumatic spinal cord injury: an overview of pathophysiology, models and acute injury mechanisms. Front Neurol 10:282

    Article  PubMed  PubMed Central  Google Scholar 

  2. Ahuja CS, Wilson JR, Nori S, Kotter MRN, Druschel C, Curt A, Fehlings MG (2017) Traumatic spinal cord injury. Nat Rev Dis Primers 3:17018

    Article  PubMed  Google Scholar 

  3. McDonough A, Monterrubio A, Ariza J, Martínez-Cerdeno V (2015) Calibrated forceps model of spinal cord compression injury. J Vis Exp 98:52318

    Google Scholar 

  4. Sharif-Alhoseini M, Khormali M, Rezaei M, Safdarian M, Hajighadery A, Khalatbari MM, Safdarian M, Meknatkhah S, Rezvan M, Chalangari M, Derakhshan P, Rahimi-Movaghar V (2017) Animal models of spinal cord injury: a systematic review. Spinal Cord 8:714–721

    Article  Google Scholar 

  5. Cheriyan T, Ryan DJ, Weinreb JH, Cheriyan J, Paul JC, Lafage V, Kirsch T, Errico TJ (2014) Spinal cord injury models: a review. Spinal Cord 52:588–595

    Article  CAS  PubMed  Google Scholar 

  6. Lee JH, Streijger F, Tigchelaar S, Maloon M, Liu J, Tetzlaff W, Kwon BK (2012) A contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor. J Vis Exp 65:3313

    Google Scholar 

  7. Stokes BT, Jakeman LB (2002) Experimental modelling of human spinal cord injury: a model that crosses the species barrier and mimics the spectrum of human cytopathology. Spinal Cord 40:101–109

    Article  CAS  PubMed  Google Scholar 

  8. Krishna V, Andrews H, Jin X, Yu J, Varma A, Wen X, Kindy M (2013) A contusion model of severe spinal cord injury in rats. J Vis Exp 78:50111

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Stem Cell & Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, India

    Syed Shadab Raza

  2. Department of Stem Cell Biology and Regenerative Medicine, Era’s Lucknow Medical College Hospital, Era University, Lucknow, India

    Syed Shadab Raza

Authors
  1. Syed Shadab Raza
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Correspondence to Syed Shadab Raza .

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Editors and Affiliations

  1. Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA

    Swapan K. Ray

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Raza, S.S. (2024). Creating a Reproducible Model of Spinal Cord Injury in Rats: A Contusion Approach. In: Ray, S.K. (eds) Neuroprotection. Methods in Molecular Biology, vol 2761. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3662-6_37

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  • DOI: https://doi.org/10.1007/978-1-0716-3662-6_37

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3661-9

  • Online ISBN: 978-1-0716-3662-6

  • eBook Packages: Springer Protocols

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