Abstract
Groundbreaking work by Takahashi and Yamanaka in 2006 demonstrated that non-embryonic cells can be reprogrammed into pluripotent stem cells (PSCs) by forcing the expression of a defined set of transcription factors in culture, thus overcoming ethical concerns linked to embryonic stem cells. Induced PSCs have since revolutionized biomedical research, holding tremendous potential also in other areas such as livestock production and wildlife conservation. iPSCs exhibit broad accessibility, having been derived from a multitude of cell types and species. Apart from humans, iPSCs hold particular medical promise in the horse. The potential of iPSCs has been shown in a variety of biomedical contexts in the horse. However, progress in generating therapeutically useful equine iPSCs has lagged behind that reported in humans, with the generation of footprint-free iPSCs using non-integrative reprogramming approaches having proven particularly challenging. A greater understanding of the underlying molecular pathways and essential factors required for the generation and maintenance of equine iPSCs and their differentiation into relevant lineages will be critical for realizing their significant potential in veterinary regenerative medicine. This article outlines up-to-date protocols for the successful culture of equine iPSC, including colony selection, expansion, and adaptation to feeder-free conditions.
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Acknowledgments
JF holds a PhD studentship funded through an industrial contract with IC BIOSOLUTIONS. The Roslin Institutes receives funding from the Biotechnology and Biological Sciences Research Council through an institute strategic program grant.
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Falk, J., Donadeu, F.X. (2024). Equine Induced Pluripotent Stem Cell Culture. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 2749. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3609-1_16
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DOI: https://doi.org/10.1007/978-1-0716-3609-1_16
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