Abstract
Research on individual viruses and phages, as well as viral populations (viromes), is greatly expanding. Phages and viromes are increasingly suspected to have numerous impacts on the ecosystem in which they reside by interacting directly or indirectly with the other organisms present in their environment. In particular, phage communities of the gut microbiota have been associated with a wide range of diseases. However, properly investigating intestinal viromes is still very challenging, both experimentally and analytically. This chapter proposes a simple and reproducible protocol to separate and enrich DNA phage particles from fecal samples, to sequence them, and finally obtain a basic but robust bioinformatic characterization and classification of the global bacteriophage community.
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Acknowledgments
We are grateful to Marie-Agnès Petit, Thania Sbaghdi, and Youzheng Teo for critical reading and review of the manuscript.
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Lamy-Besnier, Q., Garneau, J.R. (2024). Enrichment, Sequencing, and Identification of DNA Bacteriophages from Fecal Samples. In: Pantaleo, V., Miozzi, L. (eds) Viral Metagenomics. Methods in Molecular Biology, vol 2732. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3515-5_9
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DOI: https://doi.org/10.1007/978-1-0716-3515-5_9
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