Abstract
Reverse transcription quantitative PCR (RT-qPCR) allows sensitive and specific measurement of mRNA transcripts from a given sample in a short period of time. Relative and absolute RT-qPCR are two strategies that could be used to quantify mRNA transcripts, based on the goal of the experiment. Here, we describe the protocol for the quantification of plant viral RNA transcripts from an infected sample using both strategies.
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Acknowledgements
We thank the Academy of Finland (Grant 1332950) for KM and Doctoral Programme in Plant Sciences, University of Helsinki, for PD for financial support.
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Dutta, P., Mäkinen, K. (2024). Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue. In: Fontes, E.P., Mäkinen, K. (eds) Plant-Virus Interactions. Methods in Molecular Biology, vol 2724. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3485-1_7
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DOI: https://doi.org/10.1007/978-1-0716-3485-1_7
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