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Fluorescence Spectroscopy: A Useful Method to Explore the Interactions of Small Molecule Ligands with DNA Structures

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Reverse Engineering of Regulatory Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2719))

Abstract

Small molecule ligands-DNA interactions have recently received a lot of attention in the fields of life sciences, medicine, and chemical sciences. To decode these interactions, many strategies have been developed. DNA is the primary target for a wide range of drugs that may interact with DNA in particular or non-specific ways and impact its activities. Fluorescence spectroscopy is a highly advanced and non-invasive technology for measuring the concentrations of substrates and products or identifying characteristic processing states. Small molecule ligands-DNA interaction studies are beneficial not only in comprehending the method of interaction, but also in synthesizing DNA-targeted particular drugs. Several small compounds that bind to DNA are clinically established therapeutic medicines, while their specific mechanism of action is unknown. Figuring out their molecular recognizing patterns is the only way to construct innovative compounds that can target specific DNA sequences with strong affinities. This book chapter will mostly explore several fluorescence spectroscopic methodologies used to investigate interactions between small molecule ligands and DNA. In addition, we provide many approaches for determining a drug’s binding mode with DNA. These strategies produce data that is both trustworthy and easy to comprehend. All of the knowledge gained by studying these fluorescence spectroscopies are supposed to lead to the development of more efficient new pharmaceuticals that might aid in the treatment of diseases.

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Acknowledgments

Mr. Sagar Bag thanks UGC, Govt. of India for providing fellowship and research grant [UGC-JRF, NTA reference number: 201610001623]. Dr. Sudipta Bhowmik thanks DST, Govt. of India for research funding.

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Authors and Affiliations

  1. Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, West Bengal, India

    Sagar Bag & Sudipta Bhowmik

  2. Mahatma Gandhi Medical Advanced Research Institute (MGMARI), Sri Balaji Vidyapeeth (Deemed to be University), Pondicherry, India

    Sudipta Bhowmik

Authors
  1. Sagar Bag
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  2. Sudipta Bhowmik
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Correspondence to Sudipta Bhowmik .

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Editors and Affiliations

  1. Department of Electronics and Communication Engineering, Jalpaiguri Govt. Engineering College, Jalpaiguri, West Bengal, India

    Sudip Mandal

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Bag, S., Bhowmik, S. (2024). Fluorescence Spectroscopy: A Useful Method to Explore the Interactions of Small Molecule Ligands with DNA Structures. In: Mandal, S. (eds) Reverse Engineering of Regulatory Networks. Methods in Molecular Biology, vol 2719. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3461-5_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3461-5_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3460-8

  • Online ISBN: 978-1-0716-3461-5

  • eBook Packages: Springer Protocols

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