Skip to main content
SpringerLink
Log in

Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue

  • Protocol
  • First Online:
Tissue-Resident Macrophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2713))

Abstract

Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local tissue environment. Here, we present a protocol to analyze different macrophage populations from murine WAT using flow cytometry.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 229.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Rosen ED, Spiegelman BM (2014) What we talk about when we talk about fat. Cell 156:20–44

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Choe SS, Huh JY, Hwang IJ, Kim JI, Kim JB (2016) Adipose tissue remodeling: its role in energy metabolism and metabolic disorders. Front Endocrinol (Lausanne) 7:30

    Article  PubMed  Google Scholar 

  3. Chakarov S, Bleriot C, Ginhoux F (2022) Role of adipose tissue macrophages in obesity-related disorders. J Exp Med 219:e20211948

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW Jr (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112:1796–1808

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Cox N, Geissmann F (2020) Macrophage ontogeny in the control of adipose tissue biology. Curr Opin Immunol 62:1–8

    Article  CAS  PubMed  Google Scholar 

  6. Cox N, Crozet L, Holtman IR, Loyher PL, Lazarov T, White JB, Mass E, Stanley ER, Elemento O, Glass CK et al (2021) Diet-regulated production of PDGFcc by macrophages controls energy storage. Science 373:eabe9383

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We thank Kim Dressler for help with isolating the tissue. Research in the Wachten lab was supported by grants from the DFG – SFB 1454 – project number 432325352, TRR83 – 112927078, TRR333/1 – 450149205, under Germany’s Excellence Strategy – EXC2151 – 390873048 as well as intramural funding from the University of Bonn.

Author information

Authors and Affiliations

  1. Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany

    Dalila Juliana Silva Ribeiro, Seniz Yüksel & Dagmar Wachten

  2. Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany

    Andreas Dolf

Authors
  1. Dalila Juliana Silva Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seniz Yüksel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andreas Dolf
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dagmar Wachten
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dagmar Wachten .

Editor information

Editors and Affiliations

  1. Life & Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

    Elvira Mass

Rights and permissions

Reprints and permissions

Copyright information

© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Silva Ribeiro, D., Yüksel, S., Dolf, A., Wachten, D. (2024). Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue. In: Mass, E. (eds) Tissue-Resident Macrophages. Methods in Molecular Biology, vol 2713. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3437-0_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3437-0_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3436-3

  • Online ISBN: 978-1-0716-3437-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation