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Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model

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Vascular Hyperpermeability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2711))

Abstract

Vascular permeability, a tightly regulated process, is a direct measure of angiogenic and immune responses in the endothelium altered in several acute and chronic diseases such as sepsis, high-altitude pulmonary edema (HAPE), high-altitude cerebral edema (HACE), ischemia, and coronavirus disease 2019 (COVID-19) endotheliitis. Both endogenous and exogenous factors such as cytokines, chemokines, and hormones may affect vascular permeability. The conventional tools available for the measurement of vascular permeability in vitro and in vivo based on collagen-coated Transwell and dye-based spectrophotometric methods are indirect measures of permeability. In this chapter, we present our live in ovo protocols based on dextran-Texas red and avian chorioallantoic membrane assay developed using custom-made equipment to assess leakiness of endothelial cell barrier both in vitro and in vivo. Further, we validate this assay using different stressors such as ischemia and hypoxia known to affect endothelial barrier properties by potentiating actin stress fiber disorganization and disrupting the cell-cell junctions.

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Acknowledgments

The authors acknowledge the Rhode Island Foundation grant (20190594), Alzheimer administrative supplement to COBRE CPVB (P20 GM103652), and TEAM UTRA grant from Brown University to JHS.

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Authors and Affiliations

  1. Department of Molecular Pharmacology, Physiology and Biotechnology, Providence, RI, USA

    Jamila Siamwala

  2. Warren Alpert Medical School of Brown University, Providence Veterans Affairs Medical Center, Providence, RI, USA

    Jamila Siamwala

  3. Department of Biotechnology, Anna University, Chennai, India

    Akila Swaminathan & Suvro Chatterjee

  4. Department of Biotechnology, The University of Burdwan, Burdwan, India

    Akila Swaminathan & Suvro Chatterjee

Authors
  1. Jamila Siamwala
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  2. Akila Swaminathan
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  3. Suvro Chatterjee
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Corresponding author

Correspondence to Jamila Siamwala .

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Editors and Affiliations

  1. Morehouse School of Medicine, Atlanta, GA, USA

    Binu Tharakan

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Siamwala, J., Swaminathan, A., Chatterjee, S. (2024). Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model. In: Tharakan, B. (eds) Vascular Hyperpermeability. Methods in Molecular Biology, vol 2711. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3429-5_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3429-5_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3428-8

  • Online ISBN: 978-1-0716-3429-5

  • eBook Packages: Springer Protocols

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