Abstract
Understanding how motor circuits are organized and recruited in order to perform complex behavior is an essential question of neuroscience. Here we present an optogenetic protocol on larval zebrafish that allows spatial selective control of neuronal activity within a genetically defined population. We combine holographic illumination with the use of effective opsin transgenic lines, alongside high-speed behavioral monitoring to dissect the motor circuits of the larval zebrafish.
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Acknowledgments
The authors would like to acknowledge Martin Carbó-Tano for the development of the behavioral monitoring protocol including the 3D printed claw and the enucleation protocol that leads to Fig. 2. in this chapter and Mathilde Lapoix for her advice in the implementation of the behaviour protocol and the members of the Wyart Lab for feedback and discussion. We also thank Dimitrii Tanese, Nelson Rebola and Joana Guedes for proofreading of the manuscript. We thank Ben Shababo and Osnath Assayag from Intelligent Imaging for the technical support on using Phasor. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement #813457 as well as Fondation pour la Recherche Médicale (FRM, grant #EQU202003010612) and the Fondation Bettencourt-Schueller (FBS, grant #Don0063).
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Jia, X., Wyart, C. (2024). Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_8
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DOI: https://doi.org/10.1007/978-1-0716-3401-1_8
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