Abstract
Researchers can often successfully generate antibodies to predicted epitopes. Especially when the epitopes are on the surface of a protein or in a hydrophilic loop. But it is difficult to direct recombinant antibodies to bind either to- or near a specific amino acid on a protein or peptide. We have developed a unique immune-targeting strategy, that we call “Epivolve,” that enables us to make site-specific antibodies (Abs). Epivolve technology leverages a highly immunogenic modified amino acid that acts as a “pseudo-hapten” immuno-target and takes advantage of Ab affinity maturation technologies to make high-affinity site-specific antibodies. Epivolve functions by the evolution of an Ab paratope to either synonymous or especially non-synonymous amino acid (aa) binding. Here we describe the use of Epivolve technology in phage display and the protocols for developing site-specific antibodies.
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Acknowledgments
The authors would like to thank Dr. Brian K. Kay for insightful comments and discussion. This research was funded by NIH Appl. ID 1R43GM146473-01 and NIH Appl. ID 1R44AI177126-01.
Patent protection for the Epivolve technology has been submitted for Abbratech Inc.
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Li, X. et al. (2023). Epivolve: A Protocol for Site-Directed Antibodies. In: Hust, M., Lim, T.S. (eds) Phage Display. Methods in Molecular Biology, vol 2702. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3381-6_29
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DOI: https://doi.org/10.1007/978-1-0716-3381-6_29
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