Abstract
Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here we describe a method to detect poly-ubiquitination of NRLP3 using two different approaches: (i) detection with a ubiquitin antibody or (ii) using TUBEs (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLRs or other proteins.
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Acknowledgments
This work was supported by funds from the Manchester Collaborative Centre for Inflammation Research and the Sir Henry Dale fellowship from the Wellcome Trust (UK; Grant 104192/Z/14/Z).
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Worboys, J.D., Palazón-Riquelme, P., López-Castejón, G. (2023). Method to Measure Ubiquitination of NLRs. In: Pelegrín, P., Di Virgilio, F. (eds) NLR Proteins. Methods in Molecular Biology, vol 2696. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3350-2_7
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DOI: https://doi.org/10.1007/978-1-0716-3350-2_7
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