Abstract
This chapter provides guidance for introducing Cryptococcus neoformans into the zebrafish larvae model system to establish a CNS infection phenotype that mimics cryptococcal meningitis as seen in humans. The method outlines techniques for visualizing different stages of pathology development, from initial to severe infection profiles. The chapter provides tips for real time visualization of the interactions between the pathogen and different aspects of the CNS anatomy and immune system.
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Chalakova, Z.P., Johnston, S.A. (2023). Zebrafish Larvae as an Experimental Model of Cryptococcal Meningitis. In: Drummond, R.A. (eds) Antifungal Immunity. Methods in Molecular Biology, vol 2667. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3199-7_4
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DOI: https://doi.org/10.1007/978-1-0716-3199-7_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3198-0
Online ISBN: 978-1-0716-3199-7
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