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Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications

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Plant Iron Homeostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2665))

Abstract

Vital biochemical reactions including photosynthesis to respiration require iron, which should be tightly regulated. Although increasing evidence reveals the importance of epigenetic regulation in gene expression and signaling, the role of histone modifications and chromatin remodeling in plant iron homeostasis is not well understood. In this study, we surveyed publicly available ChIP-seq datasets of Arabidopsis wild-type and mutants defective in key enzymes of histone modification and chromatin remodeling and compared the deposition of epigenetic marks on loci of genes involved in iron regulation. Based on the analysis, we compiled a comprehensive list of iron homeostasis genes with differential enrichment of various histone modifications. This report will provide a resource for future studies to investigate epigenetic regulatory mechanisms of iron homeostasis in plants.

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Acknowledgments

This study was supported by Duke Kunshan University, 2022 SRS fund, 2021 Interdisciplinary Seed Grant, Synear and Wang-Cai Seed Grant 2022, and Wang-Cai Biochemistry Lab Grant to J. L.

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  1. Authors Yang Yu, Yuxin Wang, Zhujun Yao and Ziqin Wang have contributed equally to this chapter.

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  1. Division of Natural and Applied Sciences, Duke Kunshan University, Jiangsu, China

    Yang Yu, Yuxin Wang, Zhujun Yao, Ziqin Wang, Zijun Xia & Joohyun Lee

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  1. Yang Yu
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  2. Yuxin Wang
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  3. Zhujun Yao
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  4. Ziqin Wang
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Correspondence to Joohyun Lee .

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  1. Department of Biology, Amherst College, Amherst, MA, USA

    Jeeyon Jeong

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Yu, Y., Wang, Y., Yao, Z., Wang, Z., Xia, Z., Lee, J. (2023). Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications. In: Jeong, J. (eds) Plant Iron Homeostasis. Methods in Molecular Biology, vol 2665. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3183-6_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3183-6_9

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