Abstract
The method described here provides a fast and efficient way to obtain an enriched preparation of tRNA of interest, which is also posttranscriptionally modified by the intracellular machinery of the host cells, E. coli. While this preparation also contains a mixture of total E. coli tRNA, the enriched tRNA of interest is obtained in high yields (milligram) and is highly efficient for biochemical assays in vitro. It is routinely used in our lab for arginylation.
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References
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Acknowledgments
This work was supported by NIH R35GM122505 and R01NS102435 to A.K.
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Avcilar-Kucukgoze, I., Gamper, H., Hou, YM., Kashina, A.S. (2023). Preparation of an Enriched tRNAArg Fraction for Arginylation by Expression in E. coli. In: Kashina, A.S. (eds) Protein Arginylation. Methods in Molecular Biology, vol 2620. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2942-0_13
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DOI: https://doi.org/10.1007/978-1-0716-2942-0_13
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2942-0
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