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Vector Tropism

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Vectorology for Optogenetics and Chemogenetics

Part of the book series: Neuromethods ((NM,volume 195))

Abstract

Recombinant viruses expand the neurobiology toolbox and offer researchers an assortment of gene delivery options. Each type of virus varies in its capabilities and limitations, and therefore, there are important considerations in choosing the best viral vector system for each application. The suitability of a viral vector for gene delivery is dictated by factors such as genomic cargo capacity, duration and regulation of its transgene expression, immunogenicity, toxicity, and viral tropism. A myriad of permissive host factors is necessary to successfully express the viral genome and deliver transgenes. Viral tropism depends on the interaction of host and virion surface proteins for attachment and a concerted virus and host gene expression and assembly system. In this chapter, we hope to provide readers with a practical guide for selecting vectors for gene delivery in neuroscience applications.

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Author notes

  1. Shih-Heng Chen and Bo He contributed equally.

Authors and Affiliations

  1. Viral Vector Core, Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH/DHHS, Research Triangle Park, NC, USA

    Shih-Heng Chen, Bo He, Sarwyn Singh & Negin P. Martin

Authors
  1. Shih-Heng Chen
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  2. Bo He
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  3. Sarwyn Singh
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  4. Negin P. Martin
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Corresponding author

Correspondence to Negin P. Martin .

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Editors and Affiliations

  1. NIMH, National Institutes of Health, Behesda, MD, USA

    Mark A.G. Eldridge

  2. Department of Neurology, School of Medicine, and Emory National Primate Research Center, Emory University, Atlanta, GA, USA

    Adriana Galvan

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Chen, SH., He, B., Singh, S., Martin, N.P. (2023). Vector Tropism. In: Eldridge, M.A., Galvan, A. (eds) Vectorology for Optogenetics and Chemogenetics. Neuromethods, vol 195. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2918-5_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2918-5_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2917-8

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