Abstract
Flow cytometry is an essential tool for studying the tumor-immune microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. This chapter provides an overview of the flow cytometry instrumentation and a discussion of the appropriate considerations and steps in building a reproducible flow cytometry staining panel. We present an updated lymphoid tissue and solid tumor-infiltrating leucocyte flow cytometry staining protocol and an example of flow cytometry data analysis.
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Acknowledgments
This research was funded by the Canadian Institutes of Health Research grant PJT-162260 and the Canadian Cancer Society (grant #707140) to SVDR and by the Leukemia and Lymphoma Society of Canada (grant # 622895) and Cancer Research Society (grant #840423) to KKM.
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Santinon, F., Young, Y.K., del Rincón, S.V., Mann, K.K. (2023). Analyzing the Tumor-Immune Microenvironment by Flow Cytometry. In: Ursini-Siegel, J. (eds) The Tumor Microenvironment. Methods in Molecular Biology, vol 2614. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2914-7_2
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DOI: https://doi.org/10.1007/978-1-0716-2914-7_2
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