Abstract
Protonemata of the moss Physcomitrium patens are ideal structures in which to observe cytoskeletal organization and dynamics. Special care is needed to prepare P. patens cultures for high-resolution microscopy. Here, we describe methods for spinning disk microscopy of dividing P. patens cells expressing sGFP-tubulin and H2B-mCherry, including detailed methods for culturing P. patens.
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Acknowledgments
We would like to thank Takumi Kataoka (Miyagi University) for his technical assistance. This research was supported by JSPS KAKENHI (18H02471 to TM, 19K06726 to YH).
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Hiwatashi, Y., Murata, T. (2023). Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens. In: Hussey, P.J., Wang, P. (eds) The Plant Cytoskeleton. Methods in Molecular Biology, vol 2604. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2867-6_12
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DOI: https://doi.org/10.1007/978-1-0716-2867-6_12
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