Abstract
Pollen cryopreservation technique provides an inimitable and effective way to store viable pollen grains for many years. It can be used to minimize non-contemporary anthesis and extend the pollination period of onion. As a result, onion breeders, researchers, and farmers who produce mother bulbs or seeds profit the most. The family Alliaceae has wide genetic diversity, and most of the species are biennial or perennial in nature for flowering. Therefore, a pollen cryobank is found to be another source to conserve pollen, which is used for developing ordinary bulbs or other edible parts, F1 hybrids, seed setting, to restore fertility in male sterile line, as well as for hybrid seed production. This chapter covers the collection, dehydration, germination, and conservation of onion pollen. The standardized approach can be used to preserve healthy and viable pollen with a high fertility rate in a more profitable, precise, and concise way.
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Pandav, A.K., Saha, K., Rajasekharan, P.E. (2023). Onion (Allium cepa L.) Pollen Cryopreservation Protocol. In: Rajasekharan, P., Rohini, M. (eds) Pollen Cryopreservation Protocols . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2843-0_23
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DOI: https://doi.org/10.1007/978-1-0716-2843-0_23
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