Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules involved in the post-transcriptional regulation of specific mRNA targets, thus possibly controlling many biological processes. The miRNA profiling analysis can contribute to understanding several signaling pathways, as biomarkers for molecular diagnostic, as well as potential to be used as therapeutic targets. The miRNAs expression can be analyzed by quantitative reverse transcription PCR (RT-qPCR), microarrays, and RNA sequencing. The RT-qPCR method is sensitive and specific and has a lower cost when compared to other techniques as microarrays and RNA sequencing. Therefore, the protocol presented in this chapter describes step by step all the details to perform miRNA analysis using primer-based RT-qPCR.
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Acknowledgments
The authors would like to thank the National Council for Scientific and Technological Development – CNPq (grant number #420152/2018-0), Coordination for the Improvement of Higher Education Personnel (CAPES, Finance code 001), and the São Paulo Research Foundation, FAPESP (grant number #2014/22887-0; #2018/20175-3; #2019/25675-7; #2019/23840-0 #2020/08478-0; #2020/13075-2).
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de Ferronato, G.A. et al. (2023). MicroRNA Profiling Using a PCR-Based Method. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 2595. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2823-2_11
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DOI: https://doi.org/10.1007/978-1-0716-2823-2_11
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