Abstract
Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC staining workflow using specific antibodies against CD4, CD8α, FOXP3, and B220 to identify distinct lymphocyte populations including T and B cells. This staining strategy can be adapted to include other cell markers to evaluate the immune contexture in murine tissues.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Pang, L., Ernst, M., Huynh, J. (2023). Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry. In: Kalyuzhny, A.E. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 2593. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2811-9_20
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DOI: https://doi.org/10.1007/978-1-0716-2811-9_20
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2810-2
Online ISBN: 978-1-0716-2811-9
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