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Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2588))

Abstract

Saliva is a complex oral biofluid composed of various biological molecules secreted by major and minor salivary glands, as well as by-products of host oral cells, oral bacteria, small extracellular vesicles (sEVs), and gingival crevicular fluid. Recently, salivary small extracellular vesicles and salivary multi-omics (microbiome, transcriptome, DNA methylome and proteome) are emerging as potential diagnostic tools for oral diseases, including the highly prevalent periodontitis. Here, we describe the methodologies for how to isolate salivary sEVs using the size exclusion chromatography method, and how to perform salivaomics, which may guide future dental research.

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Acknowledgments

This work was supported by the Australian Dental Research Foundation (grant number 2741-2020 to PH, XL, WW, SI), the National Science Foundation of China (82001420 to XL; 82171517 to WW), and the Research Foundation of medical Sci-Tech innovation platform of Zhongnan Hospital (PTXM2021006).

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Authors and Affiliations

  1. School of Dentistry, The University of Queensland, Brisbane, Australia

    Pingping Han & Sašo Ivanovski

  2. School of Dentistry, Centre for Orofacial Regeneration, Reconstruction and Rehabilitation (COR3), The University of Queensland, Brisbane, Australia

    Pingping Han & Sašo Ivanovski

  3. Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan, China

    Xiang Li & Wei Wei

  4. Medical Research Institute, Wuhan University, Wuhan, China

    Xiang Li

  5. Department of Neurosurgery and Brain Research Center, Zhongnan Hospital, Wuhan University, Wuhan, China

    Xiang Li & Wei Wei

Authors
  1. Pingping Han
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  2. Xiang Li
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  3. Wei Wei
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  4. Sašo Ivanovski
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Corresponding author

Correspondence to Sašo Ivanovski .

Editor information

Editors and Affiliations

  1. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Gregory J. Seymour

  2. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Mary P. Cullinan

  3. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Nicholas C.K. Heng

  4. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Paul R. Cooper

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Han, P., Li, X., Wei, W., Ivanovski, S. (2023). Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics. In: Seymour, G.J., Cullinan, M.P., Heng, N.C., Cooper, P.R. (eds) Oral Biology. Methods in Molecular Biology, vol 2588. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2780-8_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2780-8_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2779-2

  • Online ISBN: 978-1-0716-2780-8

  • eBook Packages: Springer Protocols

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