Abstract
Mass spectrometry-based proteomic approaches permit the high-throughput assessment of proteins from oral biofluids, therefore, allowing a deeper insight into the mechanistic study of periodontal disease. Here we describe an entire experimental design of proteomic workflow for oral biofluids, exemplified by saliva and gingival crevicular fluid collected from periodontal health or disease subjects and using a label-free quantification strategy for mass spectrometric data acquisition.
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References
Kassebaum NJ, Smith AGC, Bernabe E, Fleming TD, Reynolds AE, Vos T et al (2017) Global, regional, and national prevalence, incidence, and disability-adjusted life years for oral conditions for 195 countries, 1990–2015: a systematic analysis for the global burden of diseases, injuries, and risk factors. J Dent Res 96(4):380–387. https://doi.org/10.1177/0022034517693566
Baumgartner D, Johannsen B, Specht M, Luddecke J, Rombach M, Hin S et al (2021) OralDisk: a chair-side compatible molecular platform using whole saliva for monitoring oral health at the dental practice. Biosensors (Basel) 11(11). https://doi.org/10.3390/bios11110423
Bostanci N, Belibasakis GN (2018) Gingival crevicular fluid and its immune mediators in the proteomic era. Periodontol 2000 76(1):68–84. https://doi.org/10.1111/prd.12154
Johannsen B, Karpisek M, Baumgartner D, Klein V, Bostanci N, Paust N et al (2021) One-step, wash-free, bead-based immunoassay employing bound-free phase detection. Anal Chim Acta 1153:338280. https://doi.org/10.1016/j.aca.2021.338280
Bostanci N, Mitsakakis K, Afacan B, Bao K, Johannsen B, Baumgartner D et al (2021) Validation and verification of predictive salivary biomarkers for oral health. Sci Rep 11(1):6406. https://doi.org/10.1038/s41598-021-85120-w
Leite FRM, Nascimento GG, Moller HJ, Belibasakis GN, Bostanci N, Smith PC et al (2021) Cytokine profiles and the dynamic of gingivitis development in humans. J Clin Periodontol. https://doi.org/10.1111/jcpe.13565
Davis IJ, Jones AW, Creese AJ, Staunton R, Atwal J, Chapple IL et al (2016) Longitudinal quantification of the gingival crevicular fluid proteome during progression from gingivitis to periodontitis in a canine model. J Clin Periodontol 43(7):584–594. https://doi.org/10.1111/jcpe.12548
Bostanci N, Selevsek N, Wolski W, Grossmann J, Bao K, Wahlander A et al (2018) Targeted proteomics guided by label-free quantitative proteome analysis in saliva reveal transition signatures from health to periodontal disease. Mol Cell Proteomics. 17(7):1392–1409. https://doi.org/10.1074/mcp.RA118.000718
Bostanci N, Heywood W, Mills K, Parkar M, Nibali L, Donos N (2010) Application of label-free absolute quantitative proteomics in human gingival crevicular fluid by LC/MS E (gingival exudatome). J Proteome Res. 9(5):2191–2199. https://doi.org/10.1021/pr900941z
Zhang Y, Fonslow BR, Shan B, Baek MC, Yates JR 3rd. (2013) Protein analysis by shotgun/bottom-up proteomics. Chem Rev 113(4):2343–2394. https://doi.org/10.1021/cr3003533
Schmidt A, Aebersold R (2006) High-accuracy proteome maps of human body fluids. Genome Biol 7(11):242. https://doi.org/10.1186/gb-2006-7-11-242
Bostanci N, Grant M, Bao K, Silbereisen A, Hetrodt F, Manoil D et al (2021) Metaproteome and metabolome of oral microbial communities. Periodontol 2000 85(1):46–81. https://doi.org/10.1111/prd.12351
Bostanci N, Silbereisen A, Bao K, Grossmann J, Nanni P, Fernandez C et al (2020) Salivary proteotypes of gingivitis tolerance and resilience. J Clin Periodontol. 47(11):1304–1316. https://doi.org/10.1111/jcpe.13358
Bostanci N, Ramberg P, Wahlander A, Grossman J, Jonsson D, Barnes VM et al (2013) Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis. J Proteome Res 12(2):657–678. https://doi.org/10.1021/pr300761e
Bostanci N, Bao K, Li X, Maekawa T, Grossmann J, Panse C et al (2018) Gingival exudatome dynamics implicate inhibition of the alternative complement pathway in the protective action of the C3 inhibitor Cp40 in nonhuman primate periodontitis. J Proteome Res 17(9):3153–3175. https://doi.org/10.1021/acs.jproteome.8b00263
Pasha S, Inui T, Chapple I, Harris S, Holcombe L, Grant MM (2018) The saliva proteome of dogs: variations within and between breeds and between species. Proteomics 18(3–4). https://doi.org/10.1002/pmic.201700293
Acknowledgments
We would like to thank Proteomics Biomedicum, Karolinska Institute, and the Functional Genomics Center Zurich for their fruitful collaboration over the years.
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Bostanci, N., Bao, K. (2023). Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry. In: Seymour, G.J., Cullinan, M.P., Heng, N.C., Cooper, P.R. (eds) Oral Biology. Methods in Molecular Biology, vol 2588. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2780-8_2
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DOI: https://doi.org/10.1007/978-1-0716-2780-8_2
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