Abstract
Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as, for instance, epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.
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References
Jewett CJ, Bertozzi RC (2010) Cu-free click cycloaddition reactions in chemical biology. Chem Soc Rev 39:1272–1279
Dadfar SMM, Sekula-Neuner S, Bog U et al (2018) Site-specific surface functionalization via microchannel cantilever spotting (μCS): comparison between azide–alkyne and thiol–alkyne click chemistry reactions. Small 14:1800131
Escorihuela J, Marcelis ATM, Zuilhof H (2015) Metal-free click chemistry reactions on surfaces. Adv Mater Interfaces 2:1500135
Wijdeven MA, Nicosia C, Borrmann A et al (2014) Biomolecular patterning of glass surfaces via strain-promoted cycloaddition of azides and cyclooctynes. RSC Adv 4:10549–10552
Dommerholt J, Rutjes FPJT, van Delft FL (2016) Strain-promoted 1,3-dipolar cycloaddition of cycloalkynes and organic azides. In: Vrabel M, Carell T (eds) Cycloadditions in bioorthogonal chemistry. Springer International Publishing, Cham
Debets MF, van der Doelen CWJ, Rutjes FPJT et al (2010) Azide: a unique dipole for metal-free bioorthogonal ligations. Chembiochem 11:1168–1184
Kuzmin A, Poloukhtine A, Wolfert MA et al (2010) Surface functionalization using catalyst-free azide−alkyne cycloaddition. Bioconjug Chem 21:2076–2085
Prim D, Rebeaud F, Cosandey V et al (2013) ADIBO-based “click” chemistry for diagnostic peptide micro-array fabrication: physicochemical and assay characteristics. Molecules 18:9833–9849
Cosandey V, Debrot F, Kaeser J et al (2012) Construction of a peptide microarray for auto-anti-body detection. Chim Int J Chem 66:803–806
Acknowledgements
This work was supported by the Institute of Life Technologies, School of Engineering, University of Applied Sciences and Arts Western Switzerland (HES-SO Valais-Wallis), in Sion, Switzerland.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Prim, D., Jović, M., Pfeifer, M.E. (2023). Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry. In: Cretich, M., Gori, A. (eds) Peptide Microarrays. Methods in Molecular Biology, vol 2578. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2732-7_4
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DOI: https://doi.org/10.1007/978-1-0716-2732-7_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2731-0
Online ISBN: 978-1-0716-2732-7
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