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Assay of GTPγS Binding in Autoradiography

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2576))

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Abstract

Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) ex vivo. Here, we describe the protocol for such a method, suitable for investigating CB1 receptor functionality in tissue slices from rodent brains.

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Author notes

  1. Authors Marina Gabaglio and Pamela Prini have been contributed equally to the chapter.

Authors and Affiliations

  1. Department of Biotechnology and Life Sciences, University of Insubria, Busto Arsizio, (VA), Italy

    Marina Gabaglio, Pamela Prini, Erica Zamberletti & Tiziana Rubino

  2. Fondazione Zardi-Gori, Milano, Italy

    Daniela Parolaro

Authors
  1. Marina Gabaglio
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  2. Pamela Prini
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  3. Erica Zamberletti
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  4. Tiziana Rubino
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  5. Daniela Parolaro
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Corresponding author

Correspondence to Tiziana Rubino .

Editor information

Editors and Affiliations

  1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Mauro Maccarrone

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gabaglio, M., Prini, P., Zamberletti, E., Rubino, T., Parolaro, D. (2023). Assay of GTPγS Binding in Autoradiography. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2728-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2727-3

  • Online ISBN: 978-1-0716-2728-0

  • eBook Packages: Springer Protocols

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