Abstract
T-cell repertoire characterization is a methodology that enables the identification of T-cell receptor (TCR) gene sequences in a T-cell population. TCR genes are composed of modular gene segments V (D) J that undergo somatic recombination, resulting in unique and unpredictable sequences that can be utilized to identify each T-cell clone. The analysis of the TCR composition in a T-cell population can give information on the biological phenomenon such as antigen-driven expansion and heterogeneity of T-cell responses. Bulk TCR analysis can give useful information on the clonality and can help track a specific clonotype over time or in different compartments, although the information about pairing cannot be provided. Single-cell TCR sequencing, on the other hand, can provide pairing information that are necessary to reconstruct the TCR and confirm antigen specificity.
Here we describe common methods to characterize T-cell repertoires based on both bulk and single-cell next-generation sequencing.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Pasetto, A., Buggert, M. (2022). T-Cell Repertoire Characterization. In: Huang, H., Davis, M.M. (eds) T-Cell Repertoire Characterization. Methods in Molecular Biology, vol 2574. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2712-9_9
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DOI: https://doi.org/10.1007/978-1-0716-2712-9_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2711-2
Online ISBN: 978-1-0716-2712-9
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