Abstract
Percutaneous coronary interventions (PCI) are the mainstay for treatment of advanced coronary disease. A majority of PCI involve deployment of a stent in the affected vascular segment. This chapter introduces the concept of using stents as a platform for delivering gene therapies to the vasculature with the overarching aim of mitigating in-stent restenosis (ISR), late stent thrombosis (LST), and neoatherosclerosis (NA), a triad of delayed complications that reduce the overall success rate of PCI. The chapter provides a detailed methodology for coatless reversible attachment of adenoviral (Ad) and adeno-associated viral (AAV) vectors to the metal stent struts along with representative in vitro and in vivo results.
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Acknowledgments
This work was supported in part by research grant R01HL137762 from the National Heart, Lung, and Blood Institute (to RJL an IF) and by the William J. Rashkind Endowment of the Children’s Hospital of Philadelphia (to RJL). Authors wish to acknowledge Ms. Susan Kerns for preparing the manuscript for publication.
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Alferiev, I.S., Chorny, M., Wilensky, R.L., Levy, R.J., Fishbein, I. (2022). Stent-Based Gene Delivery for Coronary Disease. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 2573. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2707-5_17
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DOI: https://doi.org/10.1007/978-1-0716-2707-5_17
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