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Lysosome Imaging Based on Fluorescent Carbon Dots

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2566))

Abstract

Lysosomes play key roles in different cellular processes such as autophagy, phagocytosis, and apoptosis. Lysosomal dysfunction is related to many diseases. Fluorescence lysosome staining strategy is valuable for the researches on the lysosome involvement in different pathological diagnosis. Here we describe fluorescence lysosome staining methods with carbon dots for the identification of lysosomes in living and fixed cells.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21974125), the 111 Project of Henan Province (CXJD2021001), and National 111 Project (D20003).

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Correspondence to Zhaohui Li .

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Guo, S., Sun, Y., Li, Z. (2023). Lysosome Imaging Based on Fluorescent Carbon Dots. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2675-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2674-0

  • Online ISBN: 978-1-0716-2675-7

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