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Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells

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Golgi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2557))

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Abstract

The protozoan parasite, Trypanosoma brucei, offers a simple system to study the growth and duplication of the Golgi. Cell lines stably expressing a photoactivatable GFP attached to an endogenous Golgi protein are permeabilized using digitonin. Photoactivation followed by imaging can then be used to follow the formation of the new Golgi.

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References

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Acknowledgments

Many thanks to Sevil Yavuz whose experimental work formed the basis of this chapter.

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Authors and Affiliations

  1. Laboratory of Molecular Cell Biology, University College London, London, UK

    Graham Warren

Authors
  1. Graham Warren
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Corresponding author

Correspondence to Graham Warren .

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Editors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA

    Yanzhuang Wang

  2. Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA

    Vladimir V Lupashin

  3. Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA

    Todd R. Graham

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Warren, G. (2023). Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells. In: Wang, Y., Lupashin, V.V., Graham, T.R. (eds) Golgi. Methods in Molecular Biology, vol 2557. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2639-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2639-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2638-2

  • Online ISBN: 978-1-0716-2639-9

  • eBook Packages: Springer Protocols

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