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Genotyping-by-Sequencing (GBS ) Method for Accelerating Marker-Assisted Selection (MAS) Program

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Genomics of Cereal Crops

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Abstract

Marker-assisted selection (MAS) plays a pivotal role in a breeding program where molecular DNA markers are used for phenotypic selections in crop improvement. Several markers have been used where SNPs (single-nucleotide polymorphisms) have been identified and effectively used. Next-generation sequencing (NGS) technologies have made significant changes to whole-genome sequencing revolutionizing plant breeding. Genotype by sequencing (GBS) is a rapid, cost-effective, and high-throughput method in NGS which enables genotyping of large populations with the discovery of SNPs. The GBS approach includes digestion of genomic DNA with restriction enzymes followed by ligation of barcode adapters, PCR amplification, and sequencing of the amplified DNA pool on a single lane of flow cells. This method has been developed and applied in the sequencing of multiplexed genomic samples. GBS is implemented successfully in genome-wide association study (GWAS), diversity studies, QTL mapping, genetic linkage analysis, marker discovery, and genomic selection under large-scale plant breeding programs.

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Author information

Authors and Affiliations

  1. International Crops Research Institute for Semi-Arid and Tropics, Hyderabad, India

    Laavanya Rayaprolu & Santosh P. Deshpande

  2. Cereal Crops Research Unit, US Department of Agriculture (USDA) Agricultural Research Service (ARS), Fargo, ND, USA

    Rajeev Gupta

Authors
  1. Laavanya Rayaprolu
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  2. Santosh P. Deshpande
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  3. Rajeev Gupta
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Editor information

Editors and Affiliations

  1. Sher-e-Kashmir University of Agricultura, KHUDWANI ANANTNAG, Jammu and Kashmir, India

    Shabir Hussain Wani

  2. Dalhousie University, Halifax, Canada

    Anuj Kumar

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Rayaprolu, L., Deshpande, S.P., Gupta, R. (2022). Genotyping-by-Sequencing (GBS ) Method for Accelerating Marker-Assisted Selection (MAS) Program. In: Wani, S.H., Kumar, A. (eds) Genomics of Cereal Crops. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2533-0_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2533-0_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2532-3

  • Online ISBN: 978-1-0716-2533-0

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