Abstract
A high-throughput quantitative determination of multiple light-emitting samples is a virtue of many light determination systems. In this chapter, we introduce a compact and efficient light determination system with a microsliding platform, a single-channel photomultiplier tube (PMT), and the controlling software for quantitative imaging of low-energy lights. The microsliding platform is uniquely designed to hold a multichannel microslide or an 8-lane PCR tube strip. The platform supports consecutive measurement of the multiple light samples through sliding the microslide or the PCR tube strip like a conveyor belt. We exemplify determination of multiple alkaline phosphatase samples and single-chain bioluminescent probes using this system. We also outline the mechanical specification of the system. This unique luminometer is an important addition to compact on-site quantitative light determination systems that are useful in various research fields including analytical chemistry, biology, and basic science in medicine.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Mirzoyan R, Goebel F, Hose J, Hsu CC, Ninkovic J, Paneque D, Rudert A, Teshima M (2007) Enhanced quantum efficiency bialkali photo multiplier tubes. Nucl Instrum Meth A 572(1):449–453. https://doi.org/10.1016/j.nima.2006.11.047
Conti M, Eriksson L (2016) Physics of pure and non-pure positron emitters for PET: a review and a discussion. EJNMMI Phys 3(1):8. https://doi.org/10.1186/s40658-016-0144-5
Partovi S, Kohan A, Rubbert C, Vercher-Conejero JL, Gaeta C, Yuh R, Zipp L, Herrmann KA, Robbin MR, Lee Z, Muzic RF Jr, Faulhaber P, Ros PR (2014) Clinical oncologic applications of PET/MRI: a new horizon. Am J Nucl Med Mol Imaging 4(2):202–212
Andrade RAN, Andrade SIE, Martins VL, Moreira PNT, Costa DJE, Lyra WS, Araujo MCU (2013) A flow-batch luminometer. Microchem J 108:151–155
Kim SB, Sato M, Tao H (2009) Molecular tension-indexed bioluminescent probe for determining protein-protein interactions. Bioconjugate Chem 20(12):2324–2330. https://doi.org/10.1021/Bc900330w
Acknowledgments
This work was supported in part by JSPS KAKENHI Grants: Numbers 15KK0029, 17H01215, 20 K21851, and 21H04948. We thank Dr. Simon Miller, Dr. Toshiya Senda, and Dr. Mikio Tanabe (Structural Biology Research Center, Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)) for preparation of purified FRB-A23-FKBP sample. This work was also partly supported by Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS) from the Japan Agency for Medical Research and Development (AMED) under grant numbers JP21am0101071.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kim, SB., Paulmurugan, R. (2022). One-Channel Microsliding Luminometer for Quantifying Low-Energy Bioluminescent Lights. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2525. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2473-9_28
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2473-9_28
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2472-2
Online ISBN: 978-1-0716-2473-9
eBook Packages: Springer Protocols