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Non-invasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2524))

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Abstract

Firefly luciferase (FLuc)-based in vivo optical imaging technology exerts the non-invasive monitoring of transplanted cells in experimental animal models. This chapter introduces an established cell line that stably expresses a retrovirus-delivered FLuc protein gene. The stable expression does not affect the cell morphology, proliferation, migration, and invasion abilities of the parental cells. After implantation, the bioluminescence signal of FLuc cells truly reflects cell proliferation and survival in vivo, which can provide a reliable method for dynamic detection of in vivo cell transplantation.

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Authors and Affiliations

  1. Stem Cell Biology and Therapy Laboratory, Department of Pediatric Surgery, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China

    Yang Bi, Nannan Zhang & Yun He

Authors
  1. Yang Bi
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  2. Nannan Zhang
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  3. Yun He
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Corresponding author

Correspondence to Yun He .

Editor information

Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Bi, Y., Zhang, N., He, Y. (2022). Non-invasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_23

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  • DOI: https://doi.org/10.1007/978-1-0716-2453-1_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2452-4

  • Online ISBN: 978-1-0716-2453-1

  • eBook Packages: Springer Protocols

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