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Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport

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The Nuclear Pore Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2502))

Abstract

The nuclear pore complex (NPC) functions as a gateway through which molecules translocate into and out of the nucleus. Understanding the transport dynamics of these transiting molecules and how they interact with the NPC has great potentials in the discovery of clinical targets. Single-molecule microscopy techniques are powerful tools to provide sub–diffraction limit information about the dynamic and structural details of nucleocytoplasmic transport. Here we detail single-point edge-excitation subdiffraction (SPEED) microscopy, a high-speed superresolution microscopy technique designed to track and map proteins and RNAs as they cross native NPCs.

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Acknowledgments

The project was supported by grants from the US National Institutes of Health (GM116204 and GM22552 to W.Y.). S.J.S. is supported by the United States Department of Veterans Affairs VR & E Program. Figures 2, 8, and 3 were created with FigureJ [Mutterer J and Zinck E (2013) Quick-and-clean article figures with FigureJ. Journal of microscopy 252 (1):89-91].

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Authors and Affiliations

  1. Department of Biology, Temple University, Philadelphia, PA, USA

    Steven J. Schnell, Mark Tingey & Weidong Yang

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  1. Steven J. Schnell
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  2. Mark Tingey
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  3. Weidong Yang
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Correspondence to Weidong Yang .

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Editors and Affiliations

  1. Department of Biosciences, Durham University, Durham, UK

    Martin W. Goldberg

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Schnell, S.J., Tingey, M., Yang, W. (2022). Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport. In: Goldberg, M.W. (eds) The Nuclear Pore Complex. Methods in Molecular Biology, vol 2502. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2337-4_23

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  • DOI: https://doi.org/10.1007/978-1-0716-2337-4_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2336-7

  • Online ISBN: 978-1-0716-2337-4

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