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Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH)

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Marine Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2498))

Abstract

The opportunity to map genes and noncoding DNA sequences on the chromosomes by fluorescence in situ hybridization (FISH) has greatly enhanced the potential for fish karyotyping and comparative cytogenetics. The use of FISH allowed for significant advances in our understanding of the fish genome architecture, especially when applied to the study of the repetitive component of the genome, that is generally underestimated in the bioinformatic assembly. Here we provide a step-by-step protocol for FISH of repeated sequences onto chromosomes of fish species.

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Author information

Authors and Affiliations

  1. National Research Council of Italy (CNR), Institute for the Study of the Anthropic Impacts and the Sustainability of the Marine Environment (IAS), Genoa, Italy

    Laura Ghigliotti & Eva Pisano

  2. Department of Marine and Environmental Sciences, Northeastern University, Marine Science Center, Nahant, MA, USA

    Juliette Auvinet

Authors
  1. Laura Ghigliotti
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  2. Juliette Auvinet
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  3. Eva Pisano
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Corresponding author

Correspondence to Laura Ghigliotti .

Editor information

Editors and Affiliations

  1. Institute of Biosciences and BioResources (IBBR), National Research Council, Naples, Italy

    Cinzia Verde

  2. Institute of Biosciences and BioResources (IBBR), National Research Council, Naples, Italy

    Daniela Giordano

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ghigliotti, L., Auvinet, J., Pisano, E. (2022). Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH). In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_21

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  • DOI: https://doi.org/10.1007/978-1-0716-2313-8_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2312-1

  • Online ISBN: 978-1-0716-2313-8

  • eBook Packages: Springer Protocols

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