Abstract
Patient-derived xenografts represent the gold standard in pre-clinical research models. The chicken embryo chorioallantoic membrane (CAM) is used in functional studies for studying biological processes such as blood vessel development and embryogenesis, biocompatible material testing, and more recently three-dimensional patient-derived xenograft (PDX) tumor modeling. We describe here a detailed method used to readily engraft established mouse PDX and primary patient tumor specimens on the CAM with as little as 25 mg of tissue per embryonated egg.
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Acknowledgments
This work is supported by the Patient-Derived Xenograft and Advanced in vivo Models Core Facility at Baylor College of Medicine with funding from NIH grant 2P30CA125123-14 and Cancer Prevention and Research Institute of Texas (CPRIT) grant #170691.
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Villanueva, H., Sikora, A.G. (2022). The Chicken Embryo Chorioallantoic Membrane (CAM): A Versatile Tool for the Study of Patient-Derived Xenografts. In: Vivanco, M.d. (eds) Mammary Stem Cells. Methods in Molecular Biology, vol 2471. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2193-6_11
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DOI: https://doi.org/10.1007/978-1-0716-2193-6_11
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