Abstract
Reproducible induction of gametocytes of Plasmodium falciparum in vitro is crucial for performing various experimental analyses to understand gametocyte cellular and molecular biology and immunology, and for the evaluation of antigametocidal agents and vaccine development. In this protocol, we present specific procedures for the enrichment, synchronous production and separation of developmental stages of P. falciparum gametocytes from culture-adapted field isolates.
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Acknowledgments
We thank Maha Saeed for initial training and helpful discussions. This work was supported by a UK Wellcome Trust grant (110090/Z/15/Z) to B.D and C.S.
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Dinko, B., Tackie, R., Henriques, G., Stewart, L., Sutherland, C. (2022). Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_11
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DOI: https://doi.org/10.1007/978-1-0716-2189-9_11
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