Abstract
Protoplasts are plant cells that have had their cell walls removed, which allows for a variety of cellular manipulations that are not possible within the context of intact plant tissue. Unfortunately, the removal of cell walls is not trivial and can be sensitive to cell type and cell differentiation state. Here, we describe a modified protoplasting protocol that improves isolation of viable protoplasts from the seedling maize shoot apex.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Luo C, Fernie AR, Yan J (2020) Single-cell genomics and epigenomics: technologies and applications in plants. Trends Plant Sci 25:1030–1040
Shillito RD, Carswell GK, Johnson CM, DiMaio JJ, Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Nat Biotechnol 7:581–587
Sheen J (2001) Signal transduction in maize and arabidopsis mesophyll protoplasts. Plant Physiol 127:1466–1475
Sheen J (1991) Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes. Plant Cell 3:225–245
Gao L, Shen G, Zhang L, Qi J, Zhang C, Ma C, Li J, Wang L, Malook SU, Wu J (2019) An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids. Plant Methods 15:144
Ortiz-Ramírez C, Arevalo ED, Xu X, Jackson DP, Birnbaum KD (2018) An efficient cell sorting protocol for maize protoplasts. Curr Protoc Plant Biol 3:e20072
Satterlee JW, Strable J, Scanlon MJ (2020) Plant stem-cell organization and differentiation at single-cell resolution. Proc Natl Acad Sci U S A 117:33689–33699
Xu X, Crow M, Rice BR, Li F, Harris B, Liu L, Demesa-Arevalo E, Lu Z, Wang L, Fox N, Wang X, Drenkow J, Luo A, Char SN, Yang B, Sylvester AW, Gingeras TR, Schmitz RJ, Ware D, Lipka AE, Gillis J, Jackson D (2021) Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery. Dev Cell 56:557–568
Altman A, Kaur-Sawhney R, Galston AW (1977) Stabilization of oat leaf protoplasts through polyamine-mediated inhibition of senescence. Plant Physiol 60:570–574
Albrechtova JTP, Heilscher S, Leske L, Walczysko P, Wagner E (2003) Calcium and pH patterning at the apical meristem are specifically altered by photoperiodic flower induction in Chenopodium spp. Plant Cell Environ 26:1985–1994
Acknowledgments
This work was funded by NSF grants IOS-2016021 and IOS-1238142 (to M.J.S.), as well as a Schmittau-Novak small grant (to J.W.S.)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Satterlee, J.W., Scanlon, M.J. (2022). Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue. In: Wang, K., Zhang, F. (eds) Protoplast Technology. Methods in Molecular Biology, vol 2464. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2164-6_9
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2164-6_9
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2163-9
Online ISBN: 978-1-0716-2164-6
eBook Packages: Springer Protocols